The use of deionized water is crucial for immunoassays in general. In this case, lower signal for the standards and controls was observed when non-deionized water was used. Despite the buffers containing EDTA, it seems that they may have been overloaded with ions and thus not able to chelate all of them.
MPTMAG-49K
MILLIPLEX® Mouse Pituitary Magnetic Bead Panel - Endocrine Multiplex Assay
The analytes available for this multiplex kit are: ACTH, BDNF, FSH, GH, Prolactin, TSH, LH.
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About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Prodotti consigliati
Livello qualitativo
Reattività contro le specie
mouse
Produttore/marchio commerciale
Milliplex®
assay range
accuracy: 96-101%
standard curve range: 12.2-50,000 pg/mL
(TSH)
standard curve range: 122-500,000 pg/mL
(Prolactin)
standard curve range: 2.4-10,000 pg/mL
(ACTH)
standard curve range: 2.4-10,000 pg/mL
(BDNF)
standard curve range: 24.4-100,000 pg/mL
(FSH)
standard curve range: 4.9-20,000 pg/mL
(GH)
standard curve range: 4.9-20,000 pg/mL
(LH)
inter-assay cv: <20%
intra-assay cv: <15%
tecniche
multiplexing: suitable
Metodo di rivelazione
fluorometric (Luminex xMAP)
Condizioni di spedizione
wet ice
Descrizione generale
Called the “master gland” because it controls many other endocrine glands, the pituitary secretes several key hormones that play important roles in the regulation of metabolism, growth, and reproduction. Piuitary hormones are responsible for the stimulation of the adrenal gland (ACTH), thyroid gland (TSH), ovaries and testes (FSH, LH) and breast milk production (prolactin), as well as blood pressure and the amount of water excreted by the kidneys. Through its connection with the pituitary, the hypothalamus modulates endocrine function by detecting hormone levels. Brain-Derived Neurotrophic Factor (BDNF) is a member of NGF family of neurotrophic factors which are required for differentiation and survival of specific neuronal sub-populations.
MILLIPLEX® Mouse Pituitary Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum, plasma, and tissue culture samples: ACTH, BDNF, FSH, GH, Prolactin, TSH and LH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
MILLIPLEX® Mouse Pituitary Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum, plasma, and tissue culture samples: ACTH, BDNF, FSH, GH, Prolactin, TSH and LH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
Applicazioni
- Analytes: ACTH, BDNF, FSH, GH, LH, Prolactin, TSH
- Recommended Sample Type: Mouse serum, plasma, cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: 10 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- Assay Run Time: For greater sensitivity an overnight incubation is recommended, (16-18 hours) at 2-8°C. Alternatively, incubate for 2 hours at room temperature (20-25°C).
- Research Category: Endocrine
- Research Subcategory: Metabolism
Caratteristiche e vantaggi
Design your multiplex kit by choosing available analytes within this panel.
Altre note
Sensitivity: Please see kit protocol for sensitive’s of individual biomarkers.
Note legali
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificati d'analisi (COA)
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Tomohiro Terasaka et al.
Endocrinology, 160(8), 1999-2014 (2019-06-13)
Gonadotropin secretion, which is elicited by GnRH stimulation of the anterior pituitary gonadotropes, is a critical feature of reproductive control and the maintenance of fertility. In addition, activation of the GnRH receptor (GnRHR) regulates transcription and translation of multiple factors
Thomas Blum et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(30), 15236-15243 (2019-07-10)
Dopamine neurons of the hypothalamic arcuate nucleus (ARC) tonically inhibit the release of the protein hormone prolactin from lactotropic cells in the anterior pituitary gland and thus play a central role in prolactin homeostasis of the body. Prolactin, in turn
Marina O Fernandez et al.
Endocrinology, 158(1), 121-133 (2016-11-15)
The peroxisome-proliferator activated receptor γ (PPARγ) is expressed in the hypothalamus in areas involved in energy homeostasis and glucose metabolism. In this study, we created a deletion of PPARγ brain-knockout (BKO) in mature neurons in female mice to investigate its
Karen J Tonsfeldt et al.
Journal of the Endocrine Society, 3(4), 716-733 (2019-03-25)
In rodents, the preovulatory LH surge is temporally gated, but the timing cue is unknown. Estrogen primes neurons in the anteroventral periventricular nucleus (AVPV) to secrete kisspeptin, which potently activates GnRH neurons to release GnRH, eliciting a surge of LH
Amanda Rodriguez et al.
Development (Cambridge, England), 146(23) (2019-11-11)
The number and quality of oocytes within the ovarian reserve largely determines fertility and reproductive lifespan in mammals. An oocyte-specific transcription factor cascade controls oocyte development, and some of these transcription factors, such as newborn ovary homeobox gene (NOBOX), are
Contenuto correlato
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Multiplex toxicity assays enable researchers to simultaneously measure multiple toxicity biomarkers of various organs to gain a better understanding of toxicity. Explore how MILLIPLEX® multiplex toxicity assays are advancing research on liver injury, kidney toxicity, genotoxicity, and more.
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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Was this a case of troubleshooting low MFIs across the standards and controls, with controls falling out of range due to the use of sterile non-deionized water to hydrate the standards and controls?
1 risposta-
Utile?
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In Milliplex Analyst, the concentrations are reported in two columns. One is labeled pg/ml(i) and one is labeled pg/ml. What is the difference between these values?
1 risposta-
The pg/ml values represent individual results and are not adjusted for the dilution factor. Additionally, the pg/ml values are the average results of the replicates, and if a dilution factor is present, this value will be multiplied by the dilution factor.
Utile?
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