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MABE191

Sigma-Aldrich

Anti-WHSC1/NSD2 Antibody, clone 29D1

clone 29D1, from mouse

Sinonimo/i:

Probable histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

29D1, monoclonal

Reattività contro le specie

human

tecniche

ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2bκ

N° accesso NCBI

NP_001035889

N° accesso UniProt

O96028

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... WHSC1(7468)

Descrizione generale

Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1), Nuclear SET domain containing protein 2 (NSD2) is a histone lysine methyltransferase that is deregulated in a subgroup of multiple myelomas. NSD proteins have been described to serve as oncogenes; however, little is known about their targets in transcriptional repression or activation. NSD2 is overexpressed in multiple myelomas via translocation between the NSD2 locus on chromosome 4 and the immunoglobulin locus on chromosome 14. NSD2 is under the regulatory control of this immunoglobulin locus. Further research is required to elucidate its roles and mechanisms in cancer.

Immunogeno

GST-tagged recombinant protein corresponding to human WHSC1/NSD2.

Applicazioni

Detect WHSC1/NSD2 using this Anti-WHSC1/NSD2 Antibody, clone 29D1 validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation.
Western Blot Analysis: 0.5-1 µg/mL from a representative lot detected WHSC1/NSD2 in 10 µg of HeLa nuclear extract and human placenta tissue lysate.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in transfected Gal4-reporter/293T cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in KMS11 cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Qualità

Evaluated by Western Blot in HCT116 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected WHSC1/NSD2 in 10 µg of HCT116 cell lysate.

Descrizione del bersaglio

~152 kDa observed. There are seven isoforms ranging between ~30 and ~152 kDa Different lysates may indicate different isoforms in Western Blots.

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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