MABC537

Anti-Vasohibin-1 Antibody, clone 4E12 (Azide Free)

clone 4E12, 1 mg/mL, from mouse

• Sinonimo/i: Vasohibin
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 4E12, monoclonal
• Reattività contro le specie: human
• Concentrazione: 1 mg/mL
• tecniche: activity assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_055724
• N° accesso UniProt: Q7L8A9
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... VASH1(22846)

Descrizione

Descrizione generale

Vasohibin-1 is an endothelium-derived negative feedback regulator of angiogenesis. Vasohibin-1 inhibited migration, proliferation, and network formation by ECs in culture, as well as angiogenesis in vivo, and Vasohibin-1 was selective for endothelial cells and its influence was eliminated by hypoxia or TNF-alpha interaction. Vasohibin is preferentially expressed in endothelial cells and does not appear to promote proliferation or tumor growth when over expressed. Vasohibin-1 expression is induced by VEGF expression and its effects are mediated through VEGF-R2 independent of VEGF-R1 and Vasohibin-1 may directly influence the expression of VEGF-R2. Because the inhibition effects of Vasohibin-1 are nullified by hypoxia and various cytokines it is thought that this is how tumors escape this built-in negative regulator of angiogenesis. Vasohibin-1 is highly expressed in fetal organs and the endothelial cells during development, and also expressed in the brain and placental and microvessels endothelial cells of atherosclerotic lesions in the adult.

Immunogeno

KLH-conjugated linear peptide corresponding to Human Vasohibin-1.

Applicazioni

Functional Activity Assay: A representative lot of this antibody induced cellular senescence (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).

Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 in HUVECs transfected with control siRNA, but not in HUVECs transfected with Vasohibin-1 siRNA (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).

Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in VEGF-stimulated HUVECs and GM7373 transfected cell lysate (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).

Immunohistochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in human placenta tissue (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).

Immunocytochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in HUVEC cells (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

This Anti-Vasohibin-1 Antibody, clone 4E12 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Immunocytochemistry and Activity Assay for the detection of Vasohibin-1.

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Vasohibin-1 in 10 µg of HEK293 cell lysate.

Descrizione del bersaglio

~ 41 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable