Extending the primary incubation step beyond the specified time in the kit protocol is not something that has been tested. A longer incubation time could potentially lead to decreased performance, including poor recovery or increased variability in assay characteristics. It may also result in higher nonspecific binding, leading to elevated signals in background wells and a loss of sensitivity.
HTH17MAG-14K
MILLIPLEX® Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay
Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Sinonimo/i:
Human Th17 cytokine multiplex kit, Luminex® human Th17 cytokine immunoassay, Millipore human Th17 cytokine panel
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About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Prodotti consigliati
Livello qualitativo
Reattività contro le specie
human
Produttore/marchio commerciale
Milliplex®
assay range
accuracy: 79-107%
standard curve range: 1-5000 pg/mL
(IL-10)
standard curve range: 10-40,000 pg/mL
(IFNγ)
standard curve range: 12-50,000 pg/mL
(IL-17A & IL-2)
standard curve range: 24-100,000 pg/mL
(IL-17 & IL-4)
standard curve range: 3-10,000 pg/mL
(IL-6 & TNFα)
standard curve range: 336-1,500,000 pg/mL
(IL-23)
standard curve range: 37-150,000 pg/mL
(IL-22 & TNFβ)
standard curve range: 488-2,000,000 pg/mL
(IL-17E/IL-25)
standard curve range: 49-200,000 pg/mL
(IL-28a & IL-31)
standard curve range: 5-20,000 pg/mL
(MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33)
standard curve range: 6-25,000 pg/mL
(IL-5)
standard curve range: 61-250,000 pg/mL
(GM-CSF & IL-27)
standard curve range: 7-30,000 pg/mL
(IL-13)
standard curve range: 9-35,000 pg/mL
(IL-9)
tecniche
multiplexing: suitable
Metodo di rivelazione
fluorometric (Luminex xMAP)
Condizioni di spedizione
wet ice
Descrizione generale
CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.
Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.
MILLIPLEX® Human TH17 Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.
MILLIPLEX® Human TH17 Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Specificità
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Applicazioni
- Analytes: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
- Recommended Sample type: Serum, plasma, and cell culture samples
- Recommended Sample dilution: Neat
- Assay Run Time: Overnight
- Research Category: Inflammation & Immunology
Caratteristiche e vantaggi
Design your multiplex kit by choosing available analytes within this panel.
Stoccaggio e stabilità
Recommended storage for kit components is 2 - 8°C.
Altre note
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.
Note legali
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Organi bersaglio
Respiratory Tract
Codice della classe di stoccaggio
10 - Combustible liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Contenuto correlato
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Could you please provide information on the potential effects of leaving samples on the shaker overnight for longer than 18 hours, for example, 24-30 hours instead of the recommended 16-18 hours?
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