D-Dimer is not present in plasma as it is only found in the blood after a blood clot is degraded. Therefore, D-Dimer is not naturally present in plasma unless the coagulation system has been activated, and it is commonly measured in serum only. In the plasma samples tested in-house, all samples were detectable but at the same concentration, so the data isn't reliable with plasma samples. The kit works well with serum samples, with normal ranges of 142 – 5146 ng/mL and a median of 433 ng/mL.
HCVD2MAG-67K
MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay
The analytes available for this multiplex kit are: ADAMTS13, D-Dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1, SAA.
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About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Prodotti consigliati
Livello qualitativo
Reattività contro le specie
human
Produttore/marchio commerciale
Milliplex®
assay range
accuracy: 88%
(Myoglobin)
accuracy: 90%
(P-selectin)
accuracy: 98%
(GDF-15)
standard curve range: 0.0005-2.5 ng/mL
(GDF-15)
standard curve range: 0.012-50 ng/mL
(Lipocalcin-2/NGAL)
standard curve range: 0.024-100 ng/mL
(Myoglobin & MPO)
standard curve range: 0.085-350 ng/mL
(sICAM-1)
standard curve range: 0.122-500 ng/mL
(sVCAM-1)
standard curve range: 0.244-1,000 ng/mL
(D-dimer, SAA & P-selectin)
standard curve range: 1.221-5,000 ng/mL
(ADAMTS 13)
inter-assay cv: <20%
intra-assay cv: <15%
tecniche
multiplexing: suitable
Metodo di rivelazione
fluorometric (Luminex xMAP)
Condizioni di spedizione
wet ice
Categorie correlate
Descrizione generale
Cardiovascular disease, particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO, 2010). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk factors.
MILLIPLEX® Human Cardiovascular Disease (CVD) Panel 2 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma and tissue/cell lysate and culture supernatant samples: Adamts13, D-dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1 and SAA. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cardiovascular
MILLIPLEX® Human Cardiovascular Disease (CVD) Panel 2 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma and tissue/cell lysate and culture supernatant samples: Adamts13, D-dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1 and SAA. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cardiovascular
Specificità
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Applicazioni
- Analytes: ADAMTS13, D-Dimer, GDF15, sICAM-1, Myeloperoxidase (MPO), Myoglobin, NGAL/Lipocalin-2, sP-selectin, Serum Amyloid A (SAA), sVCAM-1
- Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants and lysates
- Recommended Sample Dilution: 25 μL per well of 1:100 diluted serum or plasma; tissue/cell culture samples may require dilution in appropriate control medium
- NOTE: D-Dimer cannot be measured in plasma samples
- Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25 °C)
- Research Category: Cardiovascular Disease
- Research Subcategory: Metabolic Disorders
Caratteristiche e vantaggi
Design your multiplex kit by choosing available analytes within this panel.
Altre note
Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitive’s.
Note legali
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Warning
Indicazioni di pericolo
Classi di pericolo
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
10 - Combustible liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Contenuto correlato
Learn about biomarkers of cardiovascular disease (CVD) and discover how multiplex CVD panels, such as MILLIPLEX® multiplex assays, are being used to increase the efficiency of biomarker discovery for CVD research.
Uncover tips and tricks to multiplexing in this guide from the experts. Learn how you can enhance the power of your research with MILLIPLEX® multiplexing, including industry guidance for immunoassays, multiplexing tips, one-day MILLIPLEX® multiplex assays for limited lab time, and more.
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Can you provide more information about the statement in the Human CVD 2 Milliplex kit (HCVD2MAG-67K) that specifically mentions D-Dimer should be measured in serum, not plasma?
1 risposta-
Utile?
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Are the SAA beads in the Human CVD Panel 2 kit designed to target SAA-1?
1 risposta-
The capture antibody will detect SAA1 and SAA2. The detection will also detect SAA1 but does not detect SAA2. Neither antibody has been tested against SAA3 or SAA4.
Utile?
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