ABT1838

Anti-Claudin-16

from rabbit, purified by affinity chromatography

• Sinonimo/i: Paracellin-1, PCLN-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: canine, human
• tecniche: immunocytochemistry: suitable; immunofluorescence: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_006571
• N° accesso UniProt: Q9Y5I7
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CLDN16(10686)

Descrizione

Descrizione generale

Claudin-16 (UniProt: Q9Y5I7; also known as Paracellin-1, PCLN-1) is encoded by the CDLN1 (also known as PCLN1) gene (Gene ID: 10686) in human. Claudin-16 is a multi-pass membrane protein that span the plasma membrane 4 times, with their N- and C-termini located in the cytosol. It is selectively expressed at tight junctions of renal epithelial cells of the thick ascending limb of loop of Henle s where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of divalent cations. Claudin-16 may form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Over 20 different mutation sin CLDN1 gene have been reported that are linked to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC), a renal Mg2+ and Ca2+ wasting disorder that ultimately leads to progressive kidney failure. (Ref.: Muller D et al. (2006). J. Clin. Endocrinol. Metab. 91(8):3076-3079).

Specificità

This rabbit polyclonal antibody detects Claudin-16 in human and canine cells. It targets an epitope within 15 amino acids from the N-terminal region.

Immunogeno

Conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Claudin-16.

Epitope: extracellular domain

Applicazioni

Immunofluorescence Analysis: A representative lot detected Claudin-16 in Immunofluorescence applications (Kausalya, P.J., et. al. (2006). J Clin Invest. 116(4):878-91).

Inhibits Activity/Function: A representative lot blocked Clathrin-mediated endocytosis in MDCK cells (Muller, D., et. al. (2006). J Clin Endocrinol Metab. 91(8):3076-9).

Research Category
Cell Structure

This rabbit polyclonal Anti-Claudin-16, Cat. No. ABT1838, is a rabbit polyclonal antibody that detects Claudin-16 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Inhibition/Function studies.

Qualità

Evaluated by Immunocytochemistry in MDCK cells.

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Claudin-16 in Madin-Darby Canine Kidney Epithelial (MDCK) cells.

Descrizione del bersaglio

33.84 kDa calculated.

Stato fisico

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.54 M Tris, 0.2 M Glycine, 250 mM NaCl, pH 7.5 without azide.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash