ABS1634

Anti-LIP5

serum, from rabbit

• Sinonimo/i: Vacuolar protein sorting-associated protein VTA1 homolog, Dopamine-responsive gene 1 protein, DRG-1, LYST-interacting protein 5, LIP5, SKD1-binding protein 1, SBP1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_057569
• N° accesso UniProt: Q9NP79
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... VTA1(51534)

Descrizione

Descrizione generale

Vacuolar protein sorting-associated protein VTA1 homolog (UniProt: Q9NP79; also known as Dopamine-responsive gene 1 protein; DRG-1; LYST-interacting protein 5; LIP5; SKD1-binding protein 1; SBP1) is encoded by the VTA1 (also known as C6orf55; HSPC228; My012) gene (Gene ID 51534) in human. LIP5 is a 33.9 kDa protein involved in trafficking of the multi-vesicular body. It helps regulate the membrane fission and recycling activities of the ESCRT pathway. It performs its central regulatory function within the ESCRT pathway by binding ESCRT-III proteins through its N-terminal tandem microtubule-interacting and trafficking (MIT) domain and by binding VPS4 ATPases through its C-terminal VSL (Vta1/SBP1/LIP5) domain. These interactions allow LIP5 to coordinate ESCRT-III and VPS4 activities both during normal cellular homeostasis and also in response to interferon signaling during innate immune responses. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes. The LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4.

Specificità

Detects LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog) in HeLa cells

Immunogeno

His-tagged full length human recombinant LIP5.

Applicazioni

Detect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LIP5 in HeLa cells.

Western Blotting Analysis: A representative lot detected endogenous LIP5 co-immunoprecipiated with Myc-tagged VPS4A from HEK293T cells (Skalicky, J.J., et al. (2012). J Biol Chem. 287(52):43910-43926).

Research Category
Signaling

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected LIP5 in HeLa cell lysate.

Descrizione del bersaglio

~38 kDa observed. 33.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1