ABS1024

Anti-Angiomotin Antibody

serum, from rabbit

• Sinonimo/i: Angiomotin
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human, rat, mouse
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_001106962
• N° accesso UniProt: Q4VCS5
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... AMOT(154796)

Descrizione

Descrizione generale

Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.

Immunogeno

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of human Angiomotin.

Applicazioni

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Angiomotin Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Angiomotin.

Western Blotting Analysis: A representative lot detected Angiomotin in MDCK cell lysate (Wells, C. D., et al. (2006). Cell. 125:535-548).
Immunocytochemistry Analysis: A representative lot detected Angiomotin in MDCK cells (Wells, C. D., et al. (2006). Cell. 125:535-548).

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 cell lysate.

Descrizione del bersaglio

~150/80 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~118 kDa and ~73 kDa This high molecular weight observed could be due to high phosphorylation.

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1