ABE184

Anti-Fox2 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: RNA binding protein fox-1 homolog 2, Fox-1 homolog B, Fox-1 homolog Fxh, Hexaribonucleotide-binding protein 2, RNA-binding motif protein 9, RNA-binding protein 9
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, rat, mouse
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_444334
• N° accesso UniProt: Q8BP71
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RBFOX2(23543)

Descrizione

Descrizione generale

Fox2, also referred to as RNA binding protein fox-1 homolog 2, is an RNA binding protein involved in the regulation of tissue-specific alternative splicing. This protein interacts with the ER-alpha N-terminal activation domain, and contains a highly conserved RRM (RNA recognition motif), which associates with 5′-UGCAUGU-3′ elements. This protein has been demonstrated to regulate alternative splicing of tissue specific exons and differentially spliced exons during erythropoiesis.

Specificità

Other homologies: Bovine (98% sequence homology), Canine (98% sequence homology), Equine (98% sequence homology).

Immunogeno

MBP-tagged recombinant protein corresponding to mouse Fox2.

Applicazioni

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Fox2 in human cerebellum tissue.

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected Fox2 in A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Fox2 Antibody (Rabbit Polyclonal Antibody) validated in IHC(P), ICC, WB to detect Fox2 also known as RNA binding protein fox-1 homolog 2, Fox-1 homolog B.

Qualità

Evaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 50 ng/mL of this antibody detected Fox2 in 10 µg of C2C12 cell lysate.

Descrizione del bersaglio

~48 kDa observed. Alternative splicing can result in up to 9 isoforms at various molecular weights. Uncharacterized bands between ~60-80 kDa have been observed in some cell lysates.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
C2C12 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable