AB16527

Anti-MyD88 Antibody, CT

Chemicon^®, from rabbit

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse, human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: western blot: suitable
• N° accesso NCBI: NM_002468.3
• N° accesso UniProt: Q99836
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... MYD88(4615)

Descrizione

Specificità

The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (Muzio et al. 1997). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (Adachi et al. 1998; Medzhitov et al. 1998), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-defecient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

Immunogeno

Epitope: C-terminus

Rabbit anti-MyD88 (C-terminus) polyclonal antibody was raised against a peptide corresponding to amino acids 279 to 296 of human MyD88 (Bonnert et al. 1997), which are identical to those of mouse.

Applicazioni

Anti-MyD88 Antibody, C-terminus is an antibody against MyD88 for use in WB.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Western blot: 1:500 to 1:1000

Whole cell lysate from Jurkat cells can be used as positive control and a 35 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Stato fisico

Format: Purified

Purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable