676472

VEGF₁₆₅, Human, Recombinant, S. frugiperda

• Sinonimo/i: Vascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
• Codice UNSPSC: 12352202

Proprietà

• Saggio: ≥97% (SDS-PAGE)
• Livello qualitativo: 100
• Stato: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Impurezze: ≤1 EU/μg Endotoxin (EU/μg VEGF)
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Recombinant, human VEGF₁₆₅ (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic, and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfide-linked homodimer.

Azioni biochim/fisiol

ED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.

Ricostituzione

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Altre note

Liu, X., et al. 2001. J. Urology166, 354.
Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
Neufeld, G., et al. 1994. Prog. Growth Factor Res.5, 89.
Leung, D.W., et al. 1989. Science 246, 1306.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3