362185

N-Glycosidase F, Elizabethkingia meningosepticum

• Sinonimo/i: N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
• Numero CAS: 83534-39-8
• Classificazione EC (Enzyme Commission): 3.5.1.52 (BRENDA, IUBMB)
• Numero MDL: MFCD00131222
• Codice UNSPSC: 12352202
• NACRES: NA.54

Proprietà

• Origine biologica: bacterial (Elizabethkingia meningosepticum)
• Livello qualitativo: 100
• Coniugato: (N-linked)
• Forma fisica: liquid
• Attività specifica: ≥20,000 units/mg protein; ≥4500 units/mL
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: do not freeze
• Attività estranea: Proteases, none detected
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

Stato fisico

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Altre note

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable