251760

DAR-4M

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Sinonimo/i: DAR-4M, Diaminorhodamine-4M
• Formula empirica (notazione di Hill): C₂₅H₂₆N₄O₃
• Peso molecolare: 430.50
• Codice UNSPSC: 12352202
• NACRES: NA.25

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: solution
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: red-violet
• Fluorescenza: λ_ex ~560 nm; λ_em ~575 nm
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.

Azioni biochim/fisiol

Cell permeable: no

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Attenzione

Toxicity: Irritant (B)

Stato fisico

In DMSO.

Ricostituzione

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Altre note

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Note legali

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 188.6 °F
• Punto d’infiammabilità (°C): 87 °C