17-500M

Catch and Release v2.0 Reversible Immunoprecipitation System

• Sinonimo/i: Catch and release system, Immunoprecipitation kit
• Codice UNSPSC: 12161503
• eCl@ss: 32161000
• NACRES: NB.22

Proprietà

• Reattività contro le specie (prevista in base all’omologia): all
• Livello qualitativo: 100
• Produttore/marchio commerciale: Catch And Release; Upstate^®
• tecniche: activity assay: suitable (IP-kinase); immunoprecipitation (IP): suitable; western blot: suitable
• Condizioni di spedizione: wet ice

Descrizione

Descrizione generale

Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form



Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.

Applicazioni

Catch and Release v2.0 should not be used with proteins containing a 6-His tag.

Confezionamento

50 immunoprecipitations

Componenti

Catch and Release Wash Buffer, 10X (Cat.# 20-210)

Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)

Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)

20-285

20-216

Catch and Release Capture Tubes

Qualità

routinely evaluated by immunoprecipitation.

Stoccaggio e stabilità

All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS05,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H314,H360D,H410
• Consigli di prudenza: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classi di pericolo: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects