1.50671

SeQuant^™ ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L x I.D. 250 mm x 21.2 mm HPLC Column

• Codice UNSPSC: 41115709
• Lungh. × diam. int. colonna: 25 cm × 21.2 mm
• Dimensione particelle: 5 μm
• Gruppo funzionale matrice: ZIC-HILIC bonding phase
• Dimensione pori: 200 Å pore size
• Matrice: fully porous particle

Proprietà

• Nome del prodotto: ZIC-HILIC^™ (5µm,200Å) 250 x 21.2 mm, stainless steel column, suitable for HPLC, SeQuant^™
• Materiali: stainless steel column
• Livello qualitativo: 100
• agenzia: suitable for USP L114
• Nome Commerciale: SeQuant^™
• Caratteristiche: endcapped: no
• Produttore/marchio commerciale: SeQuant^™
• Parametri: 400 bar pressure; 70 °C max. temp.
• tecniche: HPLC: suitable; LC/MS: suitable (Prep LC); mass spectrometry (MS): suitable (High)
• Lungh. × diam. int. colonna: 25 cm × 21.2 mm
• Matrice: fully porous particle
• Gruppo funzionale matrice: ZIC-HILIC bonding phase
• Dimensione particelle: 5 μm
• Dimensione pori: 200 Å pore size
• pH di lavoro: 2-7.5
• Tecnica di separazione: hydrophilic interaction (HILIC)
• Temperatura di conservazione: no temp limit

Descrizione

Descrizione generale

SeQuant^™ ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 21.2 mm ID is the best choice for the semi-preparative separation of polar compounds.Thanks to their favorable retention and robust performance, SeQuant^™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^™ ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Risultati analitici

Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40

Note legali

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany