09-819

Anti-Lin28 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: western blot: suitable
• N° accesso NCBI: NP_078950
• N° accesso UniProt: Q9H9Z2
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... LIN28A(79727)

Descrizione

Descrizione generale

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancer. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

Specificità

Other homologies: Rat and Mouse 98% and 97% sequence homology

Immunogeno

Histidine-tagged recombinant protein corresponding to human Lin28.

Applicazioni

Anti-Lin28 Antibody is a Rabbit Polyclonal Antibody for detection of Lin28 also known as Protein lin-28 homolog A & has been validated in WB.

Qualità

Evaluated by Western Blot in NT2/D1 cell lyate.

Western Blot Analysis: 1:5,000 dilution of this antibody detected Lin28 in 10 µg of NT2/D1 cell lysate.

Descrizione del bersaglio

~28 kDa observed

Risultati analitici

Control
NT2/D1 cell lyate

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable