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05-1352

Sigma-Aldrich

Anti-Histone H2B Antibody, clone 5HH2-2A8

ascites fluid, clone 5HH2-2A8, from mouse

Sinonimo/i:

H2B, Histone H2B, histone 2, H2be, histone cluster 2, H2be, H2B histone family, member Q

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100 μL
566,00 €

566,00 €


Spedizione prevista il11 aprile 2025Dettagli


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Cambia visualizzazione
100 μL
566,00 €

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

566,00 €


Spedizione prevista il11 aprile 2025Dettagli


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

5HH2-2A8, monoclonal

Reattività contro le specie

mouse, human, rat

tecniche

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

N° accesso Genebanck

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... H2BC21(8349)
mouse ... H2Bc21(319190)

Descrizione generale

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificità

Antibody recognizes Histone H2B near the C-terminus.

Immunogeno

Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.

Applicazioni

Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Qualità

Evaluated by western blot in HepG2 cell lysate.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.

Descrizione del bersaglio

~15 kDa

Linkage

Replaces: MABE15

Stato fisico

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate

Note legali

GenBank is a registered trademark of United States Department of Health and Human Services

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Articoli

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Domande

Recensioni

Nessuna valutazione

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