If the column does not perform as expected after rehydration and running the QA test method, it should be cleaned according to the instructions. Then, the mobile phase should be equilibrated again, and the QA method should be rerun. If the column still does not perform as expected, it may not be regeneratable and will need to be replaced.
807164
TSKgel® DEAE-5PW (10 µm) HPLC Columns
L x I.D. 7.5 cm × 7.5 mm, DEAE (diethylaminoethyl) phase
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HUF 782,000.00
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HUF 782,000.00
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Product Name
TSKgel® DEAE-5PW HPLC Column, phase DEAE (diethylaminoethyl), L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
material
PEEK
product line
TSKgel®
feature
counter ion Cl-
manufacturer/tradename
Tosoh 07164
parameter
≤1 M salt concentration
≤20% organic solvent
1.2 mL/min flow rate
10-45 °C temperature
150 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
7.5 cm × 7.5 mm
matrix
polymer particle platform
matrix active group
DEAE (diethylaminoethyl) phase
particle size
10 μm
pore size
1000 Å
operating pH
2-12
separation technique
anion exchange
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What should be done before restarting the use of a DEAE column, and which buffer can be used to check if the DEAE column is still working?
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