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13358-U

Supelco

Lipid Removal Agent (LRA)

pkg of 100 g

Synonym(s):

mix moxde ion exchange resin

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100 G
HUF 57,400.00

HUF 57,400.00

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100 G
HUF 57,400.00

About This Item

EC Number:
215-710-8
UNSPSC Code:
23201100
NACRES:
SB.52

HUF 57,400.00

Please contact Customer Service for Availability
Request a Bulk Order

grade

reagent grade

Quality Level

100

form

powder

packaging

pkg of 100 g

technique(s)

LPLC: suitable

surface area

120 m2/g

matrix

Silica

matrix active group

silica

particle size

325 mesh

pore size

90 Å mean pore size

General description

Lipid removal agent is a synthetic adsorbent of crystalline calcium silicate hydrate which is used for removing lipids and endotoxin from plasma or aqueous solutions. It is also used for DNA purification to remove protein, RNA and genomic DNA.[1]

Application

  • Lipid removal agent (LRA) was used for absorption of lipids using gel permeation chromatography.[2]
  • LRA was used to isolate lipoprotein particles from coeluting proteins in collected fraction from Gel filtration chromatography.[3]

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
188901
182810
181208
176566
171841

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Toshihiro Sakurai et al.
The Journal of pharmacology and experimental therapeutics, 356(2), 341-353 (2015-11-18)
Apolipoprotein C-II (apoC-II) is a cofactor for lipoprotein lipase, a plasma enzyme that hydrolyzes triglycerides (TGs). ApoC-II deficiency in humans results in hypertriglyceridemia. We used zinc finger nucleases to create Apoc2 mutant mice to investigate the use of C-II-a, a
Stephen W C Chung et al.
Journal of chromatography. A, 1218(33), 5555-5567 (2011-07-12)
Organochlorine pesticide (OCP) residues in foods have been of concern for several decades. However, the analysis of some of the OCPs and their metabolites or derivatives, such as endrin aldehyde, endrin ketone, nonachlor, etc. in fatty foods (including foods of
John P Zhang et al.
Biotechnology progress, 21(4), 1220-1225 (2005-08-06)
A synthetic adsorbent of crystalline calcium silicate hydrate, the product LRA by Advanced Minerals Corp., has been studied for endotoxin removal from aqueous solutions. This adsorbent removes endotoxin effectively, and the removal is greatly enhanced by the presence of an
Scott M Gordon et al.
Journal of proteome research, 9(10), 5239-5249 (2010-08-20)
Plasma levels of high density lipoprotein cholesterol (HDL-C) are inversely proportional to the incidence of cardiovascular disease. Recent applications of modern proteomic technologies have identified upward of 50 distinct proteins associated with HDL particles with many of these newly discovered
Karol Kaiser et al.
Nature communications, 10(1), 1498-1498 (2019-04-04)
WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced

Articles

LRA (Lipid Removal Agent) for Biopharmaceutical Production

Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.

Questions

1–2 of 2 Questions  

  1. What is the protocol for the Lipid Removal Agent?

    1 answer

    1. The following protocol is intended for the initial evaluation of LRA:

      LRA (lipid removal agent) is a patented synthetic mineral that has a unique affinity for lipid, lipoprotein and endotoxin. It is a filterable alternative to fumed silica. The efficiency with respect to time that it takes for the LRA to interact with lipid or other molecules is dependent on the characteristics of the particular starting material. As a result, it is generally recommended that an initial sample test be performed after 1 to 2 hours of contact time and another sample be allowed overnight contact. This will give an indication of how long the LRA needs to be in contact with the fluid and whether additional contact time results in greater binding or, conversely, that lesser contact time results in increased process throughput.

      Objective: To determine from an initial screening test, if LRA can remove lipid
      or other impurities from a biological fluid.

      Test Method:
      1. Prepare two 10-mL aliquots of the test fluid into a centrifuge tube.
      2. Add 0.4-g of LRA to each sample.
      3. Gently agitate both samples at process temperature.
      4. After 1 to 2 hours, centrifuge one of the samples.
      5. Test the supernatant for lipid depletion* and retention of product of interest.
      6. Allow the other sample to agitate overnight.
      7. Centrifuge the overnight sample, and test the supernatant for lipid depletion* and retention of product of interest.

      The protocol may be optimized to suit the specific sample type and adjusted to account for the individual centrifuge used in the process.

      Helpful?

  2. where can I find information about this product? I want to know how it works? How to use it to remove excess lipids? And whether or not its addition in a serum/plasma sample would interfere with sodium measurement by indirect ISE.

    1 answer

    1. For more information regarding LRA (Lipid Removal Agent) for Biopharmaceutical Production, please see this publication: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/purification/lra-lipid-removal.

      For additional information on this product customers have been redirected to view the peer-reviewed articles for this product. Please see all related peer-reviewed documents towards the bottom of the product detail page, titled "Peer Reviewed Papers".

      Helpful?

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