T7782
Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
goat
Quality Level
conjugate
TRITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:64
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Immunoglobulin G (IgG)antibody is found in four subclasses IgG1, IgG2, IgG3 and IgG4. It has a monomeric structure. IgG is secreted by B cells and is found in blood and extracellular fluids.. Anti-Mouse IgG (Fab specific) is conjugated to tetramethylrhodamine isothiocyanate (TRITC) and then purified by gel filtration to remove free TRITC.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Conjugation of TRITC to Mouse IgG (Fab specific) antibody enables the use in immunofluorescent techniques.
Biochem/physiol Actions
IgG antibody subtype provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) antibody participates in complement fixation and opsonization.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse or human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Hua Jiang et al.
Molecular oncology, 8(2), 297-310 (2014-01-07)
Reprogramming of NK cells with a chimeric antigen receptor (CAR) proved an effective strategy to increase NK cell reactivity and recognition specificity toward tumor cells. To enhance the cytotoxicity of NK cells against CD138-positive multiple myeloma (MM) cells, we generated
Yi-Liang Miao et al.
Environmental and molecular mutagenesis, 50(8), 666-671 (2009-07-14)
Centrosomes are critically important for maintaining meiotic spindle integrity in the meiosis II (MII) stage where oocytes are arrested in most mammalian species before fertilization takes place. In women of advanced ages or during in vitro fertilization (IVF) procedures, aneuploidy
Sh Hejazi et al.
Iranian journal of parasitology, 7(2), 53-60 (2012-10-31)
Cutaneous leishmaniasis is a neglected parasitic disease, which imposes massive human distress and financial costs to the endemic countries. Better understanding of host immune response to the parasite leads to helpful strategies for disease control. Interleukin (IL)-10 and transforming growth
Sh Hejazi et al.
Iranian journal of parasitology, 7(3), 16-23 (2012-10-31)
Cutaneous leishmaniasis is a neglected parasitic disease, which imposes massive human distress and financial costs to the endemic countries. Better understanding of host immune response to the parasite leads to helpful strategies for disease control. Interleukin (IL)-10 and transforming growth
Shervin G Hoseini et al.
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 17(6), 513-518 (2013-04-30)
Cutaneous leishmaniasis (CL) is a public health problem in several endemic countries. Recent studies on mouse model and also a few clinical experiments showed that the type of immune response generated at the site of infection and especially balance between
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