SRP0241
EGFR (T790M) Active human
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym(s):
ERBB1, Epidermal growth factor receptor, HER1, PIG61, Proto-oncogene c-ErbB-1
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About This Item
UNSPSC Code:
12352200
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biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
88 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EGFR(1956)
General description
Human EGFR T790M (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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