MBD0047

Enterococcus faecium FISH probe – ATTO 488

Probe for fluorescence in situ hybridization (FISH), 20µM in water

• UNSPSC Code: 41105500
• NACRES: NA.54

Properties

• Quality Level: 200
• technique(s): FISH: suitable
• fluorescence: λ_ex 504 nm; λ_em 521 nm
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells[1]. Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples[2]. FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood[3][4] and tissue[5]), microbial ecology (solid biofilms[6], aquatic systems[7]) and plants[8]. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest. Enterococcus faecium probe specifically recognizes E. faecium cells[8],[9]. E. faecium a gram-positive bacterium and is a cause of a variety of infections, including endocarditis, urinary tract infections, prostatitis, intra-abdominal infection, cellulitis, and wound infection as well as concurrent bacteremia[10]. Studies have shown that E. faecium is highly resistant to multiple antibiotics and that vancomycin-resistant Enterococcus faecium (VRE) can be asymptomatically carried by healthy people, which can hamper hospitals’ attempts to control the spread of the bacteria[11]. Over the past two decades, Enterococcus faecium has emerged as a leading cause of multidrug-resistant enterococcal infection in the United States[12]. E. faecium has a high antibiotic-resistance with more than half of its pathogenic isolates expressing resistance to vancomycin, ampicillin, and high-levels of aminoglycosides[10].

Application

Probe for fluorescence in situ hybridization (FISH), recognizes Enterococcus faecium cells

Features and Benefits

• Visualize, identify, and isolate E. faecium cells.
• Observe native E. faecium cell populations in diverse microbiome environments.
• Specific, sensitive, and robust identification of E. faecium in bacterial mixed population.
• Specific, sensitive, and robust identification even when E. faecium is in low abundance in the sample.
• FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information on E. faecium morphology and allows to study biofilm architecture.
• Identify E. faecium in clinical samples such as tumor and brain tissues (for example in formalin-fixed paraffin-embedded (FFPE) samples), saliva and oral cavity and medical equipment such as dental implants and voice prostheses.
• The ability to detect E. faecium in its natural habitat is an essential tool for studying host-microbiome interaction.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable