Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Immunogen
Fc fragment of IgG purified from mouse.
Application
Anti-Mouse IgG (Fc specific) antibody produced in goat has been used in:
enzyme-linked immunosorbent assay (ELISA)
flow cytometry
immunoprecipitation
immunoblotting
Biochem/physiol Actions
IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. The antigen binding activity is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain. Anti-mouse IgG (Fc specific) antibody can be used to detect the optimal capture agents.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse or human samples.
Disclaimer
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