F5884

α-L-Fucosidase from bovine kidney

ammonium sulfate suspension, ≥2.0 units/mg protein (biuret)

• Synonym(s): α-L-Fucoside fucohydrolase
• Enzyme Commission number: 3.2.1.51 (BRENDA, IUBMB)
• MDL number: MFCD00130491
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: bovine kidney
• form: ammonium sulfate suspension
• specific activity: ≥2.0 units/mg protein (biuret)
• mol wt: 210-220 kDa (Subunits: 55-60 kDa)
• foreign activity: α-mannosidase and β-galactosidase ≤0.1%; β-N-acetylglucosaminidase ≤0.2%
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

α-L-fucosidases is a liposomal enzyme,[1] which belongs to glycosyl hydrolases family.[2]

Application

α-L-fucosidase from bovine kidney has been used:

• to study their ability to alter primary cell transduction[3]
• to examine the role of fucose residues of cell surface glycans in adhesion[4]
• to investigate its role during fertilization[5]

Biochem/physiol Actions

α-L-fucosidases degrades fucose-containing fucoglycoconjugates and breaks fucosidic bonds in oligosaccharides and glycoconjugates.[2][1] It acts as a tumor marker for hepatic and colorectal cancer.[1] α-L-fucosidase exhibits transglycosylation property.[6]

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-L-fucoside to p-nitrophenol and L-fucose per min at pH 5.5 at 25°C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 10 mM NaH₂PO₄ 10 mM citrate, pH 6.0

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable