E7510

Earle′s Balanced Salt Solution 10x

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Earle’s Balanced Salts, EBSS
• MDL number: MFCD00217343
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: glucose: 10.0 g/L; NaHCO₃: no; phenol red: 0.11 g/L
• shipped in: ambient

Description

General description

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.[1]

Application

Earle′s Balanced Salt Solution 10x has been used:

• as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs[2]
• in the swim-up method to prepare human sperm sample[3]
• in the steady-state medium to culture bovine primary mammary epithelial cells[1]

Features and Benefits

• Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
• Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
• When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
• Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate at 1×.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable