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E0282

Sigma-Aldrich

MaxGel™ ECM

mixture, liquid

Synonym(s):

Human Basement Membrane Extract (BME)

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1 ML
HUF 44,700.00

HUF 44,700.00

Please contact Customer Service for Availability
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1 ML
HUF 44,700.00

About This Item

UNSPSC Code:
12352207
eCl@ss:
32190102
NACRES:
NA.71

HUF 44,700.00

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

200

form

liquid

storage temp.

−70°C

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Application

MaxGel™ ECM is used for the following applications:
  • Extracellular matrix (ECM) protein binding assays[1][2]
  • Cell invasion assay[3]
  • Cell culture[4]
This product creates a three-dimensional environment that improves cellular proflieration by promoting cell growth and migration, and has been shown to aid the proliferation of many types of cells, including human embryonic stem cells, neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes, and keratinocytes.

Components

MaxGel ECM contains human extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin and a number of proteoglycans and glycosaminoglycans. This basement membrane extract reproduces the cooperative interactions between epithelia and mesenchyme that occur during the development and organotypic cell culture of skin.

Caution

MaxGel will remain stable for at least 1 year when stored at -70°C.

Legal Information

MaxGel is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ2147
SLCN0499
SLCK8873
SLCJ9762
SLCM8909

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Pyrimido[4,5-d]pyrimidin-4(1H)-one derivatives as selective inhibitors of EGFR threonine790 to methionine790 (T790M) mutants.
Tianfeng Xu et al.
Angewandte Chemie (International ed. in English), 52(32), 8387-8390 (2013-06-28)
Kang Li et al.
International journal of clinical and experimental pathology, 8(9), 10941-10946 (2015-12-01)
This study aims to explore the effects of vein subvolution combined with neural stem cells on nerve regeneration. Animal model of sciatic nerve defects was established with SD rats. A total of 63 SD rats were divided into control group
Bin He et al.
Oncology reports, 29(3), 1115-1123 (2012-12-21)
LIM and SH3 protein 1 (LASP1) is an actin-binding protein which is overexpressed in many types of cancers and plays important roles in cancer progression. however, the role of LASP1 in esophageal squamous cell carcinoma (ESCC) is still unknown. We sought
Shi Song et al.
American journal of cancer research, 8(6), 993-1007 (2018-07-24)
Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers. It has been demonstrated that aberrant expression of miRNAs plays an important role in HCC development. Here, we observed decreased miR-125b expression status in tumor samples from HCC
J Křivánek et al.
Folia biologica, 60 Suppl 1, 30-38 (2014-11-05)
Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to
View All Related Papers

Articles

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Questions

1–7 of 7 Questions  

  1. How do I handle Product E0282, MaxGel™ ECM?

    1 answer

    1. Remove the product from the freezer and thaw on ice just prior to use. Keep the product cold until coating.

      Please see the link below to review the product datasheet which includes the coating protocol:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf

      Helpful?

  2. is the Product E0282, MaxGel™ ECM from normal tissue or tumor tissue?

    1 answer

    1. This product is derived from the basement membranes of epithelial cells and fibroblasts. These are not strictly tumor cell lines as the normal genotype and phenotype are retained.

      Helpful?

  3. Can this product also be used for cell differentiation?

    1 answer

    1. The impact of extracellular matrix on cell differentiation can vary from cell type to cell type. This product is derived from human basement membrane extract that includes several components, many of which are not defined. Cell differentiation may be induced by the structural environment or other components present within the matrix. The end user would have to determine the suitability of the product based on the cell or tissue type of interest.

      Please refer to the literature for additional information. The following link may be of interest:
      https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01252-8#:~:text=Several%20studies%20have%20demonstrated%20that,a%20certain%20niche%20%5B46%5D.

      Please see the link below to review the product datasheet for this item:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf

      Helpful?

  4. How is Product E0282, MaxGel™ ECM, supplied?

    1 answer

    1. E0282 is supplied at a concentration of 1 mg/ml in DMEM.

      Helpful?

  5. Will Product E0282, MaxGel™ ECM, work for adherent cells and suspension cells?

    1 answer

    1. Yes, this product works for both cell types.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is an appropriate working concentration for Product E0282, MaxGel™ ECM?

    1 answer

    1. MaxGel™ works best undiluted or even diluted 1:2-1:4.  For a thin coat plating method, this material can be diluted as high as 1:100.

      Helpful?

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