Product No. D6046 is the fully supplemented liquid form of the original Dulbecco's formulation, offered as Product No. D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. D5546 is the same but does not contain L-glutamine.
D5523
Dulbecco′s Modified Eagle′s Medium - low glucose
With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
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10 L
HUF 13,400.00
10 X 1 L
HUF 20,300.00
50 L
HUF 53,600.00
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10 L
HUF 13,400.00
10 X 1 L
HUF 20,300.00
50 L
HUF 53,600.00
About This Item
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low
shipped in
ambient
storage temp.
2-8°C
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General description
Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.
Application
Quantity
Formulated to contain 10.0 grams of powder per liter of medium.
Reconstitution
Supplement with 3.7 g/L sodium bicarbonate.
also commonly purchased with this product
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Description
Pricing
supplement
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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Do you offer a liquid version of Dulbecco’s Modified Eagle’s Medium - low glucose?
1 answer-
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How do I store Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose, dry powder? How do I store the reconstituted medium?
1 answer-
Store the dry powdered medium at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark. The nature of supplements added may affect storage conditions and shelf life of the medium. The product label bears the lot-specific expiration date.
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How do I prepare Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
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Measure out 90% of final required volume of water. Water temperature should be 15-20 °C.While gently stirring the water, add the powdered medium. Stir until dissolved. Do NOT heat.Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2.To the solution in step 3, add 3.7 g sodium bicarbonate or 49.3 ml of sodium bicarbonate solution [7.5%w/v] for each liter of final volume of medium being prepared. Stir until dissolved.While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1N HCl or 1N NaOH is recommended.Add additional water to bring the solution to final volume.Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.Aseptically dispense medium into sterile container.
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What is the formulation of Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
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The formulation can be found on the DMEM formulation sheet at the following link.
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What sodium bicarbonate should I use with Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
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Sodium Bicarbonate powder (Product No. S5761) or Sodium Bicarbonate Solution, 7.5% (Product No. S8761) is recommended. For more information, consult the product datasheet.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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