Skip to Content
Merck
All Photos(1)

Key Documents

C-12209

Sigma-Aldrich

Human Umbilical Vein Endothelial Cells 2 (HUVEC 2)

Pooled, isolated in Growth Medium 2, 500,000 proliferating cells

Sign Into View Organizational & Contract Pricing

Select a Size

C-12209
HUF 172,000.00

HUF 172,000.00

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(1)

Select a Size

Change View
C-12209
HUF 172,000.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

HUF 172,000.00

Please contact Customer Service for Availability
Request a Bulk Order

form

aqueous solution

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

storage temp.

room temp

Application

Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord. They are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. HUVEC are isolated from pooled donors (from up to four different umbilical cords) in Endothelial Cell Growth Medium 2, which is free of ECGS and contains additional IGF and VEGF, and provided in a proliferating format. (Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.)

Quality

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P1Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

The Endothelial Cell Transwell Migration and Invasion Assay

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Related Content

Endothelial Cell Tube Formation Angiogenesis Assay

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service