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A8960

Sigma-Aldrich

L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate

≥95%

Synonym(s):

MAP, Magnesium Ascorbyl Phosphate

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HUF 47,400.00

HUF 47,400.00


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5 G
HUF 47,400.00

About This Item

Empirical Formula (Hill Notation):
C6H6Mg1.5O9P · xH2O
CAS Number:
Molecular Weight:
289.54 (anhydrous basis)
MDL number:
UNSPSC Code:
12352205
PubChem Substance ID:
NACRES:
NA.79

HUF 47,400.00


Please contact Customer Service for Availability

Request a Bulk Order

biological source

synthetic (organic)

Quality Level

Assay

≥95%

form

powder

technique(s)

HPLC: suitable

color

white to off-white

solubility

water: 50 mg/mL

storage temp.

room temp

SMILES string

O=C1O[C@@]([C@@H](O)CO)([H])C([O-])=C1OP([O-])([O-])=O.O=C2O[C@@]([C@@H](O)CO)([H])C([O-])=C2OP([O-])([O-])=O.O.[Mg+2].[Mg+2].[Mg+2]

InChI

1S/2C6H9O9P.3Mg.H2O/c2*7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13;;;;/h2*2,4,7-9H,1H2,(H2,11,12,13);;;;1H2/q;;3*+2;/p-6/t2*2-,4+;;;;/m00..../s1

InChI key

HKRNHMZODRSAPN-IXNKEUJHSA-H

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General description

L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate, also known as Magnesium Ascorbyl Phosphate (MAP), is a stable derivative of vitamin C (L-ascorbic acid). MAP is applied in various research domains, including emulsion-based experiments, biocatalytic dephosphorylation, cell differentiation, and tissue engineering. Its versatile utility extends to gene repression, transcriptional studies, and investigations related to growth factors. In cell biology, MAP demonstrates potential applications in inducing morphological changes in cultured cells, influencing cell death pathways.

Moreover, MAP plays a pivotal role in metabolomics and biochemical research. Acting as an antioxidant, it effectively scavenges free radicals, offering protection against oxidative damage. Its anti-inflammatory properties contribute to the reduction of inflammation, and studies have indicated its ability to stimulate collagen production. Additionally, MAP serves as a chelating agent, further expanding its applications in various research studies. The multifaceted nature of MAP makes it a valuable tool in scientific investigations, contributing to advancements in cell biology, metabolomics, and biochemical research.

Application

L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate has been used in mesenchymal stem cells (MSC) expansion medium to incubate scaffolds,[1] in Dulbecco′s modified eagle medium (DMEM) for culturing human umbilical cord cells[2] and mesenchymal stem cells.[3]

Biochem/physiol Actions

L-Ascorbic acid 2-phosphate (AA2P) is used in biocatalytic dephosphorylation for electric power generation and electrochemical detection assays. L-Ascorbic acid 2-phosphate may be used in cell differentiation and tissue engineering applications. Asc-2P is used in studies of gene repression, such as the repression of dihydrotestosterone-induced dickkopf-1 expression.

Features and Benefits

  • Can be used in Cell Biology, Metabolomics and Biochemical research
  • High-quality compound suitable for multiple research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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Effect of initial seeding density on human umbilical cord mesenchymal stromal cells for fibrocartilage tissue engineering
Wang L, et al.
Tissue Engineering: Part A, 15(5), 1009-1017 (2008)
Yang Mei et al.
Journal of biotechnology, 150(3), 438-446 (2010-10-05)
A recently developed protocol, "microtissue assembly" holds great promise to address the issue of limited mass transfer within engineered large tissue replacements (macrotissues), wherein small "building blocks" (microtissues) are prepared and then assembled into macrotissues. Previous studies suggested that aggregation
Adult bone marrow is a rich source of human mesenchymal `stem?cells but umbilical cord and mobilized adult blood are not
Wexler SA, et al.
British Journal of Haematology, 121(2), 368-374 (2003)
Martin L Decaris et al.
Annals of biomedical engineering, 39(4), 1174-1185 (2010-12-02)
The presentation of extracellular matrix (ECM) proteins provides an opportunity to instruct the phenotype and behavior of responsive cells. Decellularized cell-secreted matrix coatings (DM) represent a biomimetic culture surface that retains the complexity of the natural ECM. Microenvironmental culture conditions
Kejun Feng et al.
Biosensors & bioelectronics, 26(7), 3187-3191 (2011-01-18)
Here a highly sensitive electrochemical method is described for the detection of point mutation in DNA. Polymerization extension reaction is applied to specifically initiate enzymatic electrochemical amplification to improve the sensitivity and enhance the performance of point mutation detection. In

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Questions

1–10 of 13 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How long is stable at -20 or -80 degrees, if dissolved in water or media? By any chance, did you tested the stability of this product at -20 °C or -80 °C?

    1 answer
    1. Unfortunately, the solution stability of this product at freezer temperatures has not been determined. This product is soluble in water at 50 mg/ml. Stock solutions have been found to retain 85% activity for 1 week at 37 °C and 80% activity for 1 month at refrigerated temperatures.

      Helpful?

  4. To whom it may concern, I would like to know more on the biocompatibility of L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate. Could you please send me an example of certificate of analysis for this compound? Thanks a lot. S. Chatellier

    1 answer
    1. Please see the link below to access a specific lot or review a sample Certificate:
      https://www.sigmaaldrich.com/product/sigma/A8960#product-documentation

      Helpful?

  5. What type of filter membrane can be used to sterilize ascorbic acid?

    1 answer
    1. After reconstitution in water, it is recommended to sterilize the solution using a 0.22 micron PES filter, such as one of the following products, or any other dimension that better fits the end user's needs.
      https://www.sigmaaldrich.com/substance/steritopthreadedbottletopfilter1234598765

      Helpful?

  6. Is A8960 sterile? or can i find a sterile alternative?

    1 answer
    1. This is not a sterile product. Stock solutions should be filter-sterilized. The compound is soluble in water at 50 mg/mL. The solution stability has not been determined.

      Helpful?

  7. What is the shelf life/stability since purchased? In powder condition in its original container, at room temperature

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  8. Can stock solutions of this compound be frozen and retain their activity for extended periods of time?

    1 answer
    1. The product stability has not been determined in freezer temperature. Stock solutions that are stored at 5 °C are approximately 80% active after 4 weeks.

      Helpful?

  9. How long is A8960 stable in water when frozen at -20C?

    1 answer
    1. This product stability has not been determined in freezer temperature. Stocks solutions that are stored at 5 °C are approximately 80% active after 4 weeks.

      Helpful?

  10. How long is stable at -20 or -80 degrees, if dissolved in water or media ? Thinking to prepare a stock solution. Thank you

    1 answer
    1. This product is soluble in water at 50 mg/ml, forming a clear colorless to yellow solution. Stocks solutions that are stored at 5 °C are approximately 80% active after 4 weeks.

      If this product is reconstituted or diluted into media, it retains approximately 85% of its activity after 1 week at 37 °C and approximately 80% for 1 month at 5 °C.

      This product stability has not been determined at -20 °C or -80 °C.

      Helpful?

1–10 of 13 Questions  

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