A4685

Luminol sodium salt

luminescent, ≥98% (titration), powder and chunks

• Synonym(s): 3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione
• Linear Formula: C₈H₆N₃O₂Na
• CAS Number: 20666-12-0
• Molecular Weight: 199.14
• MDL number: MFCD00161491
• UNSPSC Code: 12352204
• PubChem Substance ID: 24890898
• NACRES: NA.83

Properties

• Product Name: Luminol sodium salt,
• Assay: ≥98% (titration)
• Quality Level: 300
• form: powder and chunks
• solubility: water: 50 mg/mL, clear to very slightly hazy, colorless to faint red
• SMILES string: O=C1NN([Na])C(C2=CC=CC(N)=C21)=O
• InChI: 1S/C8H7N3O2.Na/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12;/h1-3H,(H4,9,10,11,12,13);/q;+1/p-1
• InChI key: RVJVDCVIJCBUTH-UHFFFAOYSA-M

Description

Application

Luminol sodium salt has been used:

• as a component of MEBSS buffer in a bioluminescence assay to detect peroxidase activity in whole blood samples[1]
• to study protein interaction in whole-cell lysates by immunoprecipitation[2]
• for in vitro reactive oxygen species (ROS) quantification in CD45⁺ and CD45− cells[3]

Biochem/physiol Actions

Luminol is a redox-sensitive chemiluminescent compound.[4][5] It emits blue luminescence when the basic aqueous solution of luminol having oxygen atoms come in contact with oxidizing agents like potassium ferricyanide.[4] It is highly stable and is applicable in various fields such as analytical chemistry, metallurgy, biochemistry, and forensics for detecting reactive species.[5] Luminol has the potential to detect defects in neutrophils ex vivo during oxidative metabolism defects such as chronic granulomatous disease and myeloperoxidase deficiency.[5]

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves