This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
https://www.sciencedirect.com/science/article/pii/S0021925818639675
https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3Dihub
R0278
RIPA Buffer
RIPA Buffer
Synonym(s):
Radioimmunoprecipitation assay buffer
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50 ML
HUF 35,500.00
500 ML
HUF 189,000.00
HUF 35,500.00
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50 ML
HUF 35,500.00
500 ML
HUF 189,000.00
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HUF 35,500.00
Please contact Customer Service for Availability
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description
protease free
Quality Level
form
liquid
packaging
pkg of 50 mL
pkg of 500 mL
technique(s)
immunoprecipitation (IP): suitable
color
colorless
pH
7.8-8.2 (25 °C, 1 ×)
storage temp.
2-8°C
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General description
RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
Application
RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies:
- Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD).[1]
- Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells.[2]
RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™ Affinity Gels.
Features and Benefits
1 mL of the RIPA Buffer is used to lyse cells from one 100 mm culture dish (0.5 to 5 * 107 cells) of most adherent mammalian cell lines.
Minimizes non-specific protein binding interactions to ensure low background.
Other Notes
Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XIV (Authorisation List)
CAS No.
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Related Content
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
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Can I use R0278 for human protein extract for lymphocytes?
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Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?
1 answer-
RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf
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How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?
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Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Can this be used for Western blot experiments?
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Yes, this product would be suitable for western blotting.
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I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?
1 answer-
Yes, you should be able to use this product. Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).
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How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?
1 answer-
RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins. The exact formulation of C2978, however, is proprietary.
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Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?
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R0278 can be used on tissue, if it is well homogenized.
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Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?
1 answer-
This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.
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After preparing a lysate using Product R0278, RIPA Buffer, how should it be stored? And for how long can the lysate be stored?
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Lysates containing the appropriate inhibitor(s) can be stored at -70°C for up to a year.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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