E9379

Stains-All

~95% purity, powder

• Synonym(s): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Empirical Formula (Hill Notation): C₃₀H₂₇BrN₂S₂
• CAS Number: 7423-31-6
• Molecular Weight: 559.58
• Beilstein: 3865213
• EC Number: 231-047-7
• MDL number: MFCD00078788
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894694
• NACRES: NA.47

Properties

• Product Name: Stains-All, ~95%
• Assay: ~95%
• Quality Level: 200
• form: powder
• technique(s): microbe id | staining: suitable
• solubility: chloroform: 40 mg (PLUS 4.0 ml)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• InChI key: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Description

Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan.[1][2] It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.[3]

Biochem/physiol Actions

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange.[4] It also stains RNA (bluish purple) and DNA (blue).[5]

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Caution

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves