GATA-6 is a developmental transcriptional activator important in terminal differentiation and proliferation of cells destined to be associated with the heart, gut and gut related tissues and organs. GATA-6 works in concert with SEMA3C and PLXNA2 transcription factors and regulates the expression of their target genes. GATA-6 is particularly important in heart development as a number of genetic mutations within GATA-6 give rise to defective syndromes involving the heart or the vessels of the heart. Interestingly, recent research into pulmonary hypertension disease in adults points to a critical role of GATA-6 expression in endothelial cells lining the blood vessels. Healthy blood vessels express GATA-6 very strongly but expression is lost during vascular injury and markedly down regulated in the endothelial cells found in diseased vessels. Because GATA-6 is a direct transcriptional regulator or important vascular proteins such as endothelin-1 receptor, endothelial nitric oxide synthase, and cytokines such as Fractalkine, GATA-6’s role in modulating the affects of pulmonary hypertension and other vascular syndromes is a subject of much research.
Specificity
Recognizes GATA-6
Immunogen
Epitope: Region between amino acid positions 250-500.
Set of 3 peptides designed against isoform 1 located between position 250-500). Note that peptide sequences used are also present in the isoform 2.
Application
Immunocytochemistry Analysis: A representative lot of this antibody has been reported to detect GATA-6 in HeLa, 293T, Capan-2, and SH-SY5Ycells.
Research Category Epigenetics & Nuclear Function
Research Sub Category Transcription Factors
This Anti-GATA-6 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of GATA-6.
Quality
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: A 2 µg/mL dilution of this antibody detected GATA-6 in PC3cell lysate.
Target description
~ 62 kDa observed
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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