The specific isoform is not identified. The SDS PAGE reveals a molecular weight of 47 kDa for this product. However, this molecular weight includes a C-terminal 6His tag (0.8 kDa) and an N-terminal GST tag (approx. 26 kDa). The molecular weight of the remaining recombinant protein is just over 20 kDa. See the link below to review the product datasheet, which includes the sequence:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/610/13-173.pdf
13-173
MBP, recombinant
Synonym(s):
myelin basic protein
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500 μG
HUF 157,000.00
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500 μG
HUF 157,000.00
About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
bovine
Quality Level
form
liquid
mol wt
Mw ~47 kDa
manufacturer/tradename
Upstate®
concentration
1 mg/mL
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
Quality
Routinely evaluated by kinase assay
Physical form
500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
ProBond Resin chromatography
Storage and Stability
Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
Other Notes
Purity information is lot specific, please consult the certificate of analysis
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Vanessa D Stewart et al.
FEBS letters, 596(22), 2952-2973 (2022-09-15)
Myelin-associated glycoprotein (MAG) and Nogo inhibit neurite outgrowth by binding to receptors such as NgR1, PirB and LRP1, and they have also been shown to induce phosphorylation of Smad2, a key intermediate in the transforming growth factor β (TGFβ) signalling
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Which MBP isoform is product 13-173 (SKU)? Can I please have the sequence of it?
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