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T9285

Sigma-Aldrich

Tampón Tris-EDTA

100X concentrate for Northern and Southern blotting

Sinónimos:

Tampón TE solution

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About This Item

Número MDL:
MFCD00236359
Código UNSPSC:
41105319
ID de la sustancia en PubChem:
329826904
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

for molecular biology

Nivel de calidad

200

esterilidad

0.2 μm filtered

Formulario

liquid

pH

7.8-8.2 (1 × in water)

aplicaciones

sample preparation

actividad extraña

DNase, RNase, none detected

temp. de almacenamiento

room temp

cadena SMILES

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

Clave InChI

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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Descripción general

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Aplicación

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR[1][2]
  • gel electrophoresis, including Northern and Southern blotting[3]
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells[4]
  • antigen retrieval for immunohistochemistry[3]

Componentes

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCK0421
SLCJ8255
0000391230
0000390095
0000386778

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Marika Bogdani et al.
Scientific reports, 7(1), 17231-17231 (2017-12-10)
Cystic fibrosis (CF)-related diabetes (CFRD) is thought to result from beta-cell injury due in part to pancreas exocrine damage and lipofibrosis. CFRD pancreata exhibit reduced islet density and altered cellular composition. To investigate a possible etiology, we tested the hypothesis
Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents.
Espy MJ, et al.
Mayo Clinic Proceedings. Mayo Clinic, 77, 624-628 (2002)
Sarah Bøje et al.
Immunology and cell biology, 94(2), 185-195 (2015-08-14)
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
Schlenker F et al.
Analytical Chemistry, 93, 10538-10545 (2021)
Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
Ver todos los artículos relacionados

Questions

1–8 of 8 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Can this Tris-EDTA buffer solution be used in an IHC protocol for antigen retrieval?

    1 answer

    1. This product has not been tested specifically for antigen retrieval. The researcher would have to determine suitability. Please see the link below to product E1161. This product is a prepared 10X solution formulated specifically for retrieval and may be better suited the specified application:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/855/e1161pis.pdf

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  4. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

    1 answer

    1. This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

      Helpful?

  5. What does 'for molecular biology' mean?

    1 answer

    1. The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

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  6. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

    1 answer

    1. When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

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  7. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

    1 answer

    1. The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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