PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack^™, powder, 10X concentrate

• Sinónimos: 10X TE buffer
• Código UNSPSC: 12161700
• NACRES: NA.25

Propiedades

• Línea del producto: pHast Pack^™
• Formulario: powder
• condiciones de almacenamiento: (Tightly closed. Dry)
• impurezas: ≤5 ppm Heavy Metals (lead)
• color: white
• pH: 7.9—8.1
• solubilidad: soluble, clear, colorless
• trazas de catión: Fe: <10 ppm
• actividad extraña: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• temp. de almacenamiento: room temp

Descripción

Descripción general

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Aplicación

TE buffer is intended for use in:

• Nucleic acid gel electrophoresis
• storage and dilution of DNA and RNA
• DNA automated sequencing
• Immunohistochemistry

Características y beneficios

• Fast and easy Ready-to-use buffer
• Minimizes effort - no need to weigh and adjust pH
• Saves time and eliminates exposure to hazardous chemicals
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Envase

Foil pouch

Reconstitución

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Almacenamiento y estabilidad

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Información legal

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Warning
• Frases de peligro: H373
• Consejos de prudencia: P260 - P314 - P501
• Clasificaciones de peligro: STOT RE 2 Inhalation
• Órganos de actuación: Respiratory Tract
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable