Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
S6639
SSC Buffer 20× Concentrate
for Northern and Southern blotting, solution
Sinónimos:
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
About This Item
Productos recomendados
grade
for molecular biology
Quality Level
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected
storage temp.
room temp
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General description
Application
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence[1]
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA[2] and northern blotting of microRNA[3]
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon[4]
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Components
related product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
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Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Protocolos
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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