SML1661

Bafilomycin A1

from Streptomyces griseus, ≥90% (HPLC), DMSO solution, V-ATPase inhibitor

• Sinónimos: BafA1
• Fórmula empírica (notación de Hill): C₃₅H₅₈O₉
• Número de CAS: 88899-55-2
• Peso molecular: 622.83
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nombre del producto: Bafilomycin A1 Ready Made Solution, 0.16 mM in DMSO, from Streptomyces griseus
• origen biológico: Streptomyces griseus
• Nivel de calidad: 200
• Formulario: DMSO solution
• concentración: 0.16 mM in DMSO
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• cadena SMILES: O1[C@@H]([C@H]([C@@H](C[C@]1(O)[C@H]([C@H](O)[C@@H]([C@H]2OC(=O)\C(=C\C(=C\[C@H]([C@H]([C@H](C\C(=C\C=C\[C@@H]2OC)\C)C)O)C)\C)\OC)C)C)O)C)C(C)C
• InChI: 1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
• Clave InChI: XDHNQDDQEHDUTM-JQWOJBOSSA-N

Descripción

Descripción general

Bafilomycin A1, a macrolide antibiotic, belongs to the pleomacrolides family. It acts as a potent and selective inhibitor of vacuolar-type H⁺-ATPase. BafA1 can inhibit the viability of MG63 osteosarcoma cells. It can also stimulate mitochondrial dysfunction. BafA1 inhibits the proliferation of different types of cancer cells.

Aplicación

Bafilomycin A1 has been used:

• as an endosome acidification inhibitor to study the importance of endosome acidification in the extracellular vesicle uptake and cytosolic release of stably expressing NanoLuc luciferase-tagged Hsp70 (NLuc-Hsp70) in HeLa cells
• as a vacuolar-type H⁺-ATPase (V-ATPase) inhibitor to study its effects on autophagic turnover of light chain 3 β (LC3-II) in mice
• as an autophagy inhibitor to study its effects on primary rat liver sinusoidal endothelial cells (LSECs) defenestration

Acciones bioquímicas o fisiológicas

Bafilomycin A1 inhibits autophagy. It may exhibit anti-tumorigenic, anti-parasitic, and anti-neurodegenerative effects. Bafilomycin A1, found in lysosomes and endosomes prevents the acidification of these cell organelles. It also participates in blocking autophagosome-lysosome fusion and autolysosome acidification, steps necessary for maintaining the autophagic flux and cellular homeostasis.

Bafilomycin A1 is a macrolide antibiotic.

Bafilomycin A1 is a macrolide antibiotic. Bafilomycin A1 acts as a potent and selective inhibitor of vacuolar-type H⁺-ATPase.

Otras notas

Bafilomycin A1 solution is provided in concentration of 0.16 mM. Typical concentrations for use in cell culture are 50-100 nM.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): 188.6 °F - closed cup
• Punto de inflamabilidad (°C): 87 °C - closed cup