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Key Documents

S2015

Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinónimos:

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Components

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

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Referencia del producto
Descripción
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ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

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Pickering WP, et al.
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Claudia S Meier-Trummer et al.
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Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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