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Key Documents

N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

Sinónimos:

Neutralizing Buffer for Blotting Techniques

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

form

powder

technique(s)

Southern blotting: suitable

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Categorías relacionadas

General description

Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

Application

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

Components

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

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Referencia del producto
Descripción
Precios

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Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

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Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

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PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

T9285

Tampón Tris-EDTA, 100X concentrate for Northern and Southern blotting

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SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

B6185

Bionic™ Buffer, 10X Concentrate, for Northern and Southern blotting, solution

GERPN3005

ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

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Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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