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Merck

H2126

Sigma-Aldrich

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

Sinónimos:

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
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conjugado

conjugate (Glucosaminoglycan)

Nivel de calidad

tpo

Type II

Formulario

lyophilized powder

actividad específica

≥300 units/mg solid

mol peso

55 kDa

temp. de almacenamiento

−20°C

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Descripción general

Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[1] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[2]

Aplicación

Hyaluronidase from sheep testes has been used:
  • in flow cytometry[3]
  • in the isolation of putative spermatogonial stem cells (SSCs)[4]
  • to study the effects of magnetic stimulation in magnetic hydrogel properties[5]
  • in immunohistochemistry to visualize collagen type II distribution[6][7]
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[8]
  • for the degeneration of hydrogels[9]

Acciones bioquímicas o fisiológicas

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Definición de unidad

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Forma física

Lyophilized powder containing lactose

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Certificados de análisis (COA)

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Visite la Librería de documentos

Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Poldervaart MT, et al.
PLoS ONE, 12(6), e0177628-e0177628 (2017)
Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of
Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
Binsila KB, et al.
Animal Reproduction Science, 609-620 (2018)
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Otto IA, et al.
European Cells & Materials, 35, 132-150 (2018)

Artículos

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

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Protocolos

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Questions

  1. H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。

    1 answer
    1. This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparation

      Please see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdf

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