H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

• Sinónimos: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• Número de CAS: 37326-33-3
• Comisión internacional de enzimas: 3.2.1.35 (BRENDA, IUBMB)
• Número CE: 253-464-3
• Número MDL: MFCD00131351
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• conjugado: conjugate (Glucosaminoglycan)
• Nivel de calidad: 200
• tpo: Type II
• Formulario: lyophilized powder
• actividad específica: ≥300 units/mg solid
• mol peso: 55 kDa
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Aplicación

Hyaluronidase from sheep testes has been used:

• in flow cytometry
• in the isolation of putative spermatogonial stem cells (SSCs)
• to study the effects of magnetic stimulation in magnetic hydrogel properties
• in immunohistochemistry to visualize collagen type II distribution
• to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
• for the degeneration of hydrogels

Acciones bioquímicas o fisiológicas

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Definición de unidad

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Forma física

Lyophilized powder containing lactose

Otras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)