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H6254

Sigma-Aldrich

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

Sinónimos:

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

Número de CAS:
37326-33-3
Comisión internacional de enzimas:
3.2.1.35 (BRENDA, IUBMB)
Número CE:
253-464-3
Número MDL:
MFCD00131351
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

sheep testis

Nivel de calidad

200

tpo

Type V

Formulario

lyophilized powder

actividad específica

≥1,500 units/mg solid

mol peso

55 kDa

aplicaciones

diagnostic assay manufacturing

temp. de almacenamiento

−20°C

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Descripción general

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Aplicación

Hyaluronidase has been used:
  • as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
  • in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
  • in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Acciones bioquímicas o fisiológicas

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Definición de unidad

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Otras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000314670
0000304048
0000303070
0000309505
0000307328

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Isolation of Sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function
Anway MD, et al.
Biology of Reproduction, 68(3), 996-1002 (2003)
Human umbilical cord stromal stem cell express CD10 and exert contractile properties
Farias VA, et al.
Placenta, 32(1), 86-95 (2011)
Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis
Chao KL, et al.
Biochemistry, 46(23), 6911-6920 (2007)
Cartilage-specific matrix protein chondromodulin-I is associated with chondroid formation in salivary pleomorphic adenomas: immunohistochemical analysis
Kusafuka K, et al.
The American Journal of Pathology, 158(4), 1465-1472 (2001)
Samia Rahman et al.
Frontiers in cell and developmental biology, 9, 703038-703038 (2021-08-13)
Articular cartilage of the knee joint is avascular, exists under a low oxygen tension microenvironment, and does not self-heal when injured. Human infrapatellar fat pad-sourced mesenchymal stem cells (IFP-MSC) are an arthroscopically accessible source of mesenchymal stem cells (MSC) for
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Protocolos

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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