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Merck

SE100

Sigma-Aldrich

Silver Enhancer Kit

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About This Item

Código UNSPSC:
12171500
NACRES:
NA.47

Formulario

liquid

Nivel de calidad

uso

sufficient for ≥100 slides staining and fixing

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

2-8°C

Aplicación

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Características y beneficios

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Envase

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principio

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Solo componentes del kit

Referencia del producto
Descripción

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • S5020Silver enhancer solution A 100 mLSDS

  • S5145Silver enhancer solution B 100 mLSDS

Pictogramas

Health hazardEnvironment

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Visite la Librería de documentos

Yuan Chen et al.
Oncotarget, 9(41), 26556-26571 (2018-06-15)
Tumor hypoxia is a well-recognized driver of resistance to traditional cancer therapies such as chemotherapy and radiation therapy. We describe development of a new nanoconstruct composed of gold nanorods (GNRs) conjugated to carbonic anhydrase IX (CAIX) antibody that specifically binds
F Liu et al.
The Plant cell, 13(5), 1063-1078 (2001-05-08)
Some plant cytoplasms express novel mitochondrial genes that cause male sterility. Nuclear genes that disrupt the accumulation of the corresponding mitochondrial gene products can restore fertility to such plants. The Texas (T) cytoplasm mitochondrial genome of maize expresses a novel
Joanna Seiffert et al.
Respiratory research, 17(1), 85-85 (2016-07-21)
The increasing use of silver nanoparticles (AgNPs) in consumer products is concerning. We examined the potential toxic effects when inhaled in Brown-Norway (BN) rats with a pre-inflammatory state compared to Sprague-Dawley (SD) rats. We determined the effect of AgNPs generated
Catarina Coutinho et al.
Analytical and bioanalytical chemistry, 411(9), 1807-1824 (2018-11-06)
MicroRNAs (miRNAs) are small regulatory RNAs, the dysregulation of which has been associated with the progression of several human diseases, including cancer. Interestingly, these molecules can be used as biomarkers for early disease diagnosis and can be found in a
A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
Hadiyah N
International journal of nanomedicine (2014)

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