The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
11191
Histopaque®-1119
Histopaque®-1119
sterile-filtered, density: 1.119
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
esterilidad
sterile-filtered
Nivel de calidad
Formulario
liquid
caducidad
Expiry date on the label.
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
densidad
1.119
aplicaciones
hematology
histology
temp. de almacenamiento
2-8°C
Descripción general
Histopaque®-1119 es un medio estéril, comprobado para endotoxinas y listo para usar. Esta disolución consiste en polisacarosa y diatrizoato de sodio, ajustados a una densidad de 1,119 g/ml y a pH de 8,8 a 9,0.
Aplicación
Histopaque®-1119 es adecuado para utilizarlo junto con Histopaque-1077, ya que facilita la rápida recuperación de células mononucleares viables y de granulocitos de pequeños volúmenes de sangre completa o de médula ósea.
Características y beneficios
Histopaque®-1119 ofrece numerosas ventajas, como:
- Recuperación óptima de células viables
- Permite la separación selectiva de los tipos de células sanguíneas
- Evita la distorsión celular
- Preserva la viabilidad celular
- Mínima interferencia de células extrañas
- Rendimiento reproducible de lote a lote
- Esterilización mediante filtración y probado para endotoxinas
- Estable a 2-8°C durante al menos 2 años cuando está protegido de la luz
Principio
El proceso implica la formación de un gradiente doble al colocar una capa de igual volumen de Histopaque-1077 sobre el Histopaque-1119. La sangre completa anticoagulada se coloca con cuidado en capas sobre el medio Histopaque-1077 superior. Tras la centrifugación, la polisacarosa agrega los eritrocitos que, por tanto, sedimentan rápidamente. Los granulocitos se encuentran en la interfaz Histopaque-1077/1119 inferior, mientras que los linfocitos y otras células mononucleares se encuentran en la interfaz plasma/Histopaque-1077 superior. La mayoría de las plaquetas extrañas que se encuentran en la interfaz superior se quitan mediante centrifugación a baja velocidad durante los pasos de lavado. La contaminación con eritrocitos es insignificante.
Información legal
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Protocolos
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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Is there a product similar to Mono-Poly resolving media?
1 answer-
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
1 answer-
The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
1 answer-
Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 answer-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
1 answer-
This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05Helpful?
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The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
1 answer-
Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 answer-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
1 answer-
No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
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When using Product 11191, Histopaque®-1119, what is the expected percent recovery for the band containing neutrophils.
1 answer-
No percent recovery data has been generated. Due to the importance of technique with the Histopaque 1119-1, percent recovery varies with the skill of the operator.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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