The product is not tested for RNase or DNase. Please see the link below to review Triton X-100 product options that include RNase and DNase testing:
https://www.sigmaaldrich.com/product/compare?term=triton%20x-100%20nuclease&focus=products&page=1&perpage=30&sort=relevance&type=product&products=T8787%3ASIGMA%3Asial%2C648466%3AMM%3Asial&returnUrl=%2Fsearch%2Ftriton-x-100-nuclease
X100
Triton™ X-100
Triton™ X-100
laboratory grade
Sinónimos:
t-Octilfenoxipolietoxietanol, Éter polietilenglicol terc-octilfenil
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About This Item
Fórmula lineal:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Número de CAS:
Beilstein:
2315025
Número MDL:
Código UNSPSC:
12161900
ID de la sustancia en PubChem:
NACRES:
NA.21
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
synthetic
Nivel de calidad
grado
laboratory grade
descripción
non-ionic
Formulario
liquid
mol peso
micellar avg mol wt 80,000
average mol wt 625
número de agregación
100-155
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C (43 °F)
temperatura de transición
cloud point 65 °C
pour point ~7 °C
solubilidad
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB
13.5
cadena SMILES
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
Clave InChI
HNLXNOZHXNSSPN-UHFFFAOYSA-N
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Descripción general
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[3][4]
Aplicación
Acciones bioquímicas o fisiológicas
Tensioactivo no iónico ampliamente utilizado para la recuperación de los componentes de la membrana bajo condiciones desnaturalizantes suaves.
Características y beneficios
- Ideal for Cell Biology and Biochemical research applications
- Non-ionic nondenaturing detergent
- Superior wetting agent and emulsifier
- Highly versatile surfactant
Otras notas
For additional information on our range of Biochemicals, please complete this form.
Información legal
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
483.8 °F - closed cup
Punto de inflamabilidad (°C)
251 °C - closed cup
Equipo de protección personal
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Artículos
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Protocolos
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
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Is this product nuclease-free?
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How long is Triton X-100 stable at -80 degrees in buffer?
1 answer-
At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.
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If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?
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Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
https://www.sigmaaldrich.com/product/sigma/t8787
https://www.sigmaaldrich.com/product/mm/648466
https://www.sigmaaldrich.com/product/sigma/93443Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What are the storage conditions for the Triton 100x? It's not in the COA
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This product may be be stored in room temperature.
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Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?
1 answer-
Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.
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Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?
1 answer-
The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.
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What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?
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CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.
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What are the NFPA hazard numbers for this?
1 answer-
With the implementation of the Globally Harmonized System (GHS) of hazard classification in our safety data sheets (SDS), we decided to no longer maintain the National Fire Protection Association (NFPA) information in section 15 of the SDS. Therefore, we no longer provide updates to the NFPA ratings. Kindly review the SDS available at this link: https://www.sigmaaldrich.com/US/en/sds/sial/x100?userType=anonymous
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