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预设计siRNA

按基因搜索

MISSION®预设计siRNA系与默克公司独家合作，采用独有的Rosetta Inpharmatics siRNA设计算法创建。Rosetta siRNA设计算法利用位点特异性打分矩阵和种子区域知识，预测目标基因最具效率和特异性的序列。该算法的规则基于超过三年的基因沉默实验实证数据开发。

产品优势

  • 同类产品之中的佼佼者，基因沉默效果有保障
  • 有效敲低低丰度信使
  • 使用 11 阳性对照 siRNA 简化转染优化
  • 使用 8 阴性对照 siRNA 区分序列特异性沉默和非特异性效应
  • 数百种经过功能验证的预设计siRNA

产品特性

  • 物种：人类、小鼠和大鼠
  • 规格：2 (10 nmol)、5 (25 nmol)和10 (50 nmol) OD
  • 纯化方法：脱盐或HPLC
  • 序列形式：具有 dTdT 突出端的 21mer 双链体
  • 质检方法：100%质谱分析*
  • 形式：以管装干粉形式提供

*部分生产基地可能采用PAGE方法评估siRNA双链体。

 产品库

一种流行的混合形式是 4 种 5 nmol 双链，混合到一支管中（20 nmol 混合）；另一种形式也是 4 种 5 nmol 双链，但每种单管装（20 nmol 单装）。即便如此，我们先进的液体处理器还允许更多组合。如需针对您特定的需求进行可行性评定，请电邮sirnarequest@sial.com。

经验证的siRNA

许多常见基因靶标已经过 ≥75% mRNA 敲低验证（图 1提供了数据示例以及常订购的经验证siRNA清单表（按基因符号）。经验证的siRNA适于转染优化或用作阳性对照。

用30 nM 浓度的预设计siRNA 转染 HeLa 细胞。

图 1.用30 nM 浓度的预设计siRNA 转染 HeLa 细胞。转染后 48 小时，通过 qPCR 测定剩余基因表达水平的百分比（相对于模拟物）。数据为四份生物学重复的均值。

材料
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精选引用文献

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如果需要其他帮助，请通过oligotechserv@sial.com咨询我们的技术服务组。

MISSION是德国默克(Merck KGaA, Darmstadt, Germany)及/或其附属公司的商标。标签许可证。

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