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Merck

TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF254s

TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF254s application for TLC

材料

附屬品

产品编号
说明
价格

瓶形喷雾器

capacity 240 mL

矩形 TLC 展开罐,完整

L × H × W 27.0 cm × 26.5 cm × 7.0 cm

Sigma 薄层色谱喷雾盒

Spectroline®Spectroline E 系列紫外灯

output8 W, AC/DC input 115 V AC

矩形 TLC 展开罐,完整

L × H × W 7.5 cm × 15.5 cm × 8.0 cm

Hamilton® 薄层色谱注射器

1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),

coated tip

Spectroline®Spectroline E 系列紫外灯

output6 W, AC/DC input 120 V AC

Hamilton® 薄层色谱注射器

1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Hamilton® 薄层色谱注射器

1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),

coated tip

矩形 TLC 展开罐,完整

L × H × W 17.5 cm × 16.0 cm × 8.2 cm

Spectroline®Spectroline E 系列紫外灯

output6 W, AC/DC input 230 V AC

Spectroline®Spectroline E 系列紫外灯

output4 W, AC/DC input 230 V AC

Spectroline®Spectroline E 系列紫外灯

output8 W, AC/DC input 230 V AC

Latch-lid 薄层色谱展开槽

For use with 10 cm × 10 cm plates

Hamilton® 薄层色谱注射器

1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Latch-lid 薄层色谱展开槽

For use with 20 cm × 20 cm plates

Spectroline®Spectroline E 系列紫外灯

output4 W, AC/DC input 115 V AC

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

capacity 20 μL, accuracy: ±0.5%

用于薄层层析板的试剂喷雾器

volume 250 mL, Bottle-Type

CONDITIONS

plate

HPTLC pre-coated plate RP-18 wF 254s with concentrating zone (1.13124.0001)

eluent

[A] methanol; [B] acetonitrile; [C] water; (30:70:5, A:B:C)

migration distance

6 cm

chamber

normal chamber without chamber saturation

sample

5 μL

Dipped in MnCl2-sulfuric acid heated up to 110 C for 10 min in situ evaluation (with TLC/HPTLC scanner (Camag) UV 366 nm)