TLC Analysis Of A Natural Product From Cosmetical Matrix on HPTLC Silica gel 60 NH2 F254s

sample preparation

Standard was diluted in 10 mL isopropanol, sample was directly applied to the plate by CAMAG ATS4 6 mm bandwise.

plate

1.15161 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151161.0001)

eluent

[A] methanol; [B] acetonitrile; (9:1, A:B)

migration distance

6 cm

chamber

normal chamber without chamber saturation

sample

0.150 μL

detector

UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression) (CMS mass spectrometer (+)-ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA.)