HPLC Analysis of Xanthines on Purospher®STAR RP-C18 endcapped
CONDITIONS
column
PurospherSTAR RP-C18 endcapped, 10 cm x 3.0 mm, 3 μm particles (1.50398.0001)
mobile phase
[A] 0.1% formic acid in water [B] 0.1% formic acid in methanol
gradient
2.5% [B] held for 2 min; to 40% [B] in 10 min
flow rate
0.6 mL/min
pressure
2500 psi (170 bar)
column temp.
35 °C
detector
UV, 270 nm
injection
1 μL
sample
50 μg/mL in water:methanol (60:40)
说明
分析報告
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stimulants and as bronchodilators, notably in the treatment of asthma symptoms. This application shows the efficient separation of several common xanthines and may be applied their analysis in any number of desired matrices.
法律資訊
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany