HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles
CONDITIONS
column
Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles (Z777018)
mobile phase
150 mM potassium phosphate monobasic, pH 7.0 (adjusted with potassium hydroxide)
flow rate
0.7 mL/min
pressure
890 psi (61.4 bar)
column temp.
25 °C
detector
UV, 214 nm
injection
1 μL
sample
1 g/L each (except uracil, 0.1 g/L ) in mobile phase A
说明
分析報告
This application demonstrates the suitability of Zenix SEC-150 for the separation of proteins. Separation is by inclusion into the pore volume with larger molecules being excluded and therefore eluting earlier.
法律資訊
Zenix is a registered trademark of Sepax Technologies